Changes in the expression of HLA-DR on lymphocyte subpopulations of spouses having children with sporadic congenital heart defects without chromosomal diseases, under the influence of female’s autoserum

This study is aimed to investigate the effect of female autoserum on HLA-DR expression in various subpopulations lymphocytes obtained from spouses with children sporadic congenital heart defects without chromosomal diseases. 78 married couples disease were included group. The control group was formed 35 healthy children. immune response a mixed culture evaluated by an increased relation spontaneous cultures lymphocytes. Primary staining and male monoclonal antibodies CD45 conjugated fluorescent dyes (PC-5 PC-7) performed assess ones vice versa. activating all simultaneously occurred significantly less frequently compared control. characterized domination positive for lymphocyte. For having molecule its membrane, blocking more expressed Thus, manifested own lymphocytes, but not spouse.